Fenofibrate Ameliorates Hepatic Ischemia/Reperfusion Injury in Mice: Involvements of Apoptosis, Autophagy, and PPAR-α Activation

Figure 3

Fenofibrate attenuated IR-induced apoptosis. (a) After 8 h reperfusion, liver tissues were stained by TUNEL and observed under microscopy (magnification, 200x). Final evaluations were made using Image-Pro Plus 6.0 software to calculate the percentage of positive cells. (b) Relative Bax, Bcl-2, Caspase-3, and Caspase-9 mRNA levels were determined by qRT-PCR. (c) Western blot analysis of Bax, Bcl-2, cleaved Caspase-3, and cleaved Caspase-9 levels. The western blot results were quantified with ImageJ 8.0 software. (d) Bax and Bcl-2 protein expressions in liver tissues at 8 hours after reperfusion are shown by immunohistochemical staining (200x). Final evaluations were made using Image-Pro Plus 6.0 software to calculate the IOD of the positive staining area. Data are presented as (; for IR vs. sham; ^# for IR+FF (50) vs. IR; ⁺ for IR+FF (100) vs. IR; ^ for IR+FF (50) vs. IR+FF (100)) (reproduced from Ji et al. [19]).

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