Investigation of PPARβ/δ within Human Dental Pulp Cells: A Preliminary In Vitro Study

Figure 2

GW0742-activated PPARβ/δ represses cytokine gene expression in LPS-stimulated hDPCs: (a) treatment with GW0742 (0.01, 0.1, and 1.0 μM) did not alter hDPC viability. (b) Real-time qPCR analyses of IL6, IL1β, and TNFα in cultured hDPCs pretreated with GW0742 (for 24 h) and added LPS (2 μg/mL) for 4 h before harvesting. GW0742 significantly reduced LPS-induced IL6 and IL1β at 0.1 μM and LPS-induced IL6, IL1β, and TNFα at 1.0 μM. (c) Pretreatment with 0.01 μM GW0742 (for 24 h) significantly reduced Il6 and Tnfα mRNA levels in LPS-stimulated RAW264.7 cells (for 24 h) (; ^#vs. control; vs. LPS by Kruskal–Wallis and post hoc Dunn’s test; ). .

(a)

(b)

(c)