Research Article
Fenofibrate Protects Cardiomyocytes from Hypoxia/Reperfusion- and High Glucose-Induced Detrimental Effects
Figure 5
Fenofibrate (10 μM) increased antioxidant defense in primary cultures of cardiomyocytes subjected to hypoxia/reperfusion (H/R), high glucose (HG), or the combination of conditions (HG+H/R). Protein expression by Western blot and densitometric analysis of (a) SOD Cu2+/Zn2+, (b) SOD Mn2+, (c) catalase, (d) Nrf2, (e) Keap1, and (f) total antioxidant capacity. ND = not detectable; DMSO = dimethylsulfoxide (0.1%); Feno = fenofibrate (10 μM). # vs. control-DMSO; 5 vs. H/R-DMSO; & vs. HG-DMSO; $ vs. HG+H/R-DMSO. Values represent the of 6 different experiments. Two-way analysis of variance (ANOVA) followed by Tukey’s post hoc test.
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