α-Bisabolol Mitigates Colon Inflammation by Stimulating Colon PPAR-γ Transcription Factor: In Vivo and In Vitro Study

Figure 4

Effect of bisabolol on proinflammatory cytokines and mediator protein and mRNA expression. DSS administration significantly elevated proinflammatory cytokine concentrations. α-Bisabolol treatment significantly prevented the increase in proinflammatory cytokines at the protein (IL-6 (a), IL-1β (b), TNF-α (c), and IL-17A (d)) and mRNA ((IL-6 (e), IL-1β (f), TNF-α (g), and IL-17A (h)) levels. DSS administration also increased COX-2 and iNOS protein levels. α-Bisabolol treatment significantly downregulated protein expression (i, j). DSS administration significantly increased tissue nitrite levels, and α-bisabolol treatment prevented this increase (k). The density of immunoblots normalized to GAPDH. Eight () animals used for ELISA, six () animals used for mRNA, and six () animals used for Western blot and tissue nitrite level measurements. The data is expressed as . , , and . ns: not significant.

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