α-Bisabolol Mitigates Colon Inflammation by Stimulating Colon PPAR-γ Transcription Factor: In Vivo and In Vitro Study

Figure 5

Effect of bisabolol on colon MAPK, NF-κB signaling, PPAR-γ, PPAR-α, PPAR-β, protein, and mRNA expression. DSS administration significantly increased phosphorylation of ERK (a), JNK (b), p38 (c), and pNF-κBp65 (d) proteins. Bisabolol treatment significantly prevented the increase in the phosphorylation of these proteins. DSS administration significantly reduced expression of PPAR-γ at both the protein and mRNA levels. Bisabolol treatment significantly increased the PPAR-γ expression at both the protein and mRNA levels (e, f). PPAR-α (g, h) and PPAR-β (i, j) were not affected by DSS or bisabolol treatment at either the protein and mRNA levels. The density of immunoblots was normalized to GAPDH. Five () animals used for Western blot and mRNA measurements. The data expressed as . , , and .ns: not significant.