α-Bisabolol Mitigates Colon Inflammation by Stimulating Colon PPAR-γ Transcription Factor: In Vivo and In Vitro Study

Figure 7

Effect of bisabolol on TNF-α challenged HT-29 colonic adenocarcinoma cell proinflammatory cytokine mRNA expression and PPAR-gamma promoter assay. Lower concentrations of α-bisabolol had no affect on HT-29 cell viability, but higher concentrations and longer time had a negative impact on cell viability (a). α-Bisabolol significantly decreased the expression of proinflammatory cytokine mRNA [CXCL-1 (b) and IL-8 (c)] in TNF-α challenged HT-29 cells. The full-length sequence of PPAR-γ was cloned into PPRE-pNL1.3 [secNluc] plasmid transfected using lipofectamine LTX in HT-29 cells. α-Bisabolol(40 μM), GW1929 [PPARγ agonist (1 μM)], or GW9662 [PPARγ antagonist (1 μM)] compared to PPRE-pNL1.3 [secNluc] transfected plasmid controls. Luciferase expression at 24 h was determined in 50 μL supernatant culture media using a luminometer. Four () separate experiments carried out to obtain data points. The data expressed . , , , and . ns: not significant.

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