PPARβ/δ Agonism with GW501516 Increases Myotube PGC-1α Content and Reduces BCAA Media Content Independent of Changes in BCAA Catabolic Enzyme Expression

Figure 3

Cell content during Seahorse assay. Effect of GW501516 (GW) at 1 μM for 24 hours from Seahorse metabolic assay on relative nuclei content. Data were analyzed using student’s t-test performed using individual replicates per treatment condition and repeated across two independent experiments with per group in the final analyses. Representative images were taken using the 20× objective with red line indicating 150 μm.