PPARβ/δ Agonism with GW501516 Increases Myotube PGC-1α Content and Reduces BCAA Media Content Independent of Changes in BCAA Catabolic Enzyme Expression

Table 2

Summary of qRT-PCR primers from Integrated DNA Technologies (Coralville, IA).

Gene abbreviation	Forward sequence	Reverse sequence
Atp5b	5-AGGCCCTTTGCCAAGCTT-3	5-TTCTCCTTAGATGCAGCAGAGTACA-3
Bcat2	5-CGGACCCTTCATTCGTCAGA-3	5-CCATAGTTCCCCCCCAACTT-3
Bckdha	5-CCAGGGTTGGTGGGATGAG-3	5-GGCTTCCATGACCTTCTTTCG-3
Cebp	5-GTGTGCACGTCTATGCTAAACCA-3	5-GCCGTTAGTGAAGAGTCTCAGTTTG-3
Cox5a	5-GCTGCATCTGTGAAGAGGACAAC-3	5-CAGCTTGTAATGGGTTCCACAGT-3
Cs	5-TGAGAGGCATGAAGGGACTTGTGT-3	5-ATCTGTCCAGTTACCAGCAGCCAA-3
Fat (CD36)	5-TAGTAGAACCGGGCCACGTA-3	5-CAGTTCCGATCACAGCCCAT-3
Slc2a4 (Glut4)	5-GATGAGAAACGGAAGTTGGAGAGA-3	5-GCACCACTGCGATGATCAGA-3
Hibadh	5-GCAGCGGTGTGTTCTAGGTC-3	5-ACACGTCATAGAGGATGAGTGG-3
Ldha	5-GGCTTGTGCCATCAGTATCT-3	5-CCCGCCTAAGGTTCTTCATTAT-3
Ldhb	5-AGTCTCCCGTGCATCCTCAA-3	5-AGGGTGTCCGCACTCTTCCT-3
Nrf1	5-ACCCTCAGTCTCACGACTAT-3	5-GAACACTCCTCAGACCCTTAAC-3
Pdh	5-GAAGGCCCTGCATTCAACTTC-3	5-ATAGGGACATCAGCACCAGTGA-3
Ppargc1a	5-GACAATCCCGAAGACACTACAG-3	5-AGAGAGGAGAGAGAGAGAGAGA-3
Ppara	5-CTCGCGTGTGATAAAGC-3	5-CGATGCTGTCCTCCTTG-3
Ppard	5-GCCTCGGGCTTCCACTAC-3	5-AGATCCGATCGCACTTCTCA-3
Pparg	5-TTCAGCTCTGGGATGACCTT-3	5-CGAAGTTGGTGGGCCAGAAT-3
Scd1	5-CATCGCCTGCTCTACCCTTT-3	5-GAACTGCGCTTGGAAACCTG-3
Srebp1	5-ATCGCAAACAAGCTGACCTG-3	5-AGATCCAGGTTTGAGGTGGG-3
Tbp	5-GGGATTCAGGAAGACCACATA-3	5-CCTCACCAACTGTACCATCAG-3
Tfam	5-GAAGGGAATGGGAAAGGTAGAG-3	5-ACAGGACATGGAAAGCAGATTA-3

Abbreviations: Atp5b: ATP synthase F1; Bcat2: branched-chain aminotransferase 2, Bckdha: branched-chain alpha-keto acid dehydrogenase; Cebpa: CCAAT/enhancer-binding protein alpha; Cox5a: cytochrome C oxidase Subunit 5A; Cs: citrate synthase; Fat or CD36: fatty acid translocase; Slc2a4 or Glut4: glucose transporter 4; Hibadh: 3-hydroxyisobutyrate dehydrogenase; Ldha: lactate dehydrogenase a; Ldhb: lactate dehydrogenase b; Nrf1: nuclear respiratory factor 1; Pdh: pyruvate dehydrogenase; Ppargc1a: peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Pk1: pyruvate kinase 1; Ppara: peroxisome proliferator-activated receptor alpha; Ppard: peroxisome proliferator-activated receptor delta; Pparg: peroxisome proliferator-activated receptor gamma; Scd1: stearoyl-CoA desaturase; Srebp1: sterol regulatory element-binding protein; Tbp: TATA box binding protein; and Tfam: mitochondrial transcription factor A.