PPARβ/δ Agonism with GW501516 Increases Myotube PGC-1α Content and Reduces BCAA Media Content Independent of Changes in BCAA Catabolic Enzyme Expression

Table 3

Summary of primary antibodies used for western blot experiments.

Protein target	Type	Dilution	Company	Item	Approx. Mol Wt.	Product link
ACAD9	RP	1 : 1000	SC Biotechnology	sc-135148	68kd	Datasheet
pAkt (Ser 473)	RP	1 : 1000	SC Biotechnology	sc-7985-R	62kd	p-Akt1/2/3 (Ser 473)
Akt	RP	1 : 1000	Cell Signaling	9272	62kd	Akt Antibody#9272
β-Actin	RP	1 : 1000	SC Biotechnology	sc-130656	43kd	Datasheet
BCAT2	RP	1 : 1000	Bioss	BS-6589R	44kd	Datasheet
BCKDHa	RP	1 : 1000	ProSci	31-325	50kd	BCKDHA Antibody
pBCKDHa (Ser 293)	RP	1 : 1000	AbCam	ab200577	50kd	Phospho BCKDHA (S293)
CPT1b	RP	1 : 1000	SC Biotechnology	sc-20670	75kd	Datasheet
CS	MM	1 : 1000	SC Biotechnology	sc-390693	52kd	sc-390693
ERRα	RP	1 : 1000	SC Biotechnology	sc-66882	53kd	Datasheet
LAT1	RP	1 : 500	NOVUS Biological	NBP3-09988	47kd	Datasheet
NRF1	RP	1 : 1000	SC Biotechnology	sc-33771	68kd	Datasheet
PGC-1α	RP	1 : 1000	SC Biotechnology	sc-13067	90kd	PGC-1α (H-300)
PPARβ/δ	RP	1 : 1000	SC Biotechnology	sc-7197	52kd	Datasheet
PPARγ	RP	1 : 1000	SC Biotechnology	sc-7196	52kd	Datasheet
SREBP1	RP	1 : 1000	SC Biotechnology	sc-366	68kd	Datasheet
TFAM	RP	1 : 1000	SC Biotechnology	sc-28200	25kd	Datasheet
UCP1/2/3	RP	1 : 1000	SC Biotechnology	sc-28766	33kd	Datasheet

Abbreviations: ACAD9: Acyl-coA dehydrogenase 9; BCAT2: branched-chain aminotransferase 2; BCKDHE1α: branched-chain alpha-keto acid dehydrogenase E1α; CPT1b: carnitine palmitoyl transferase; CS: citrate synthase; ERRα: estrogen-related receptor alpha; LAT1: large amino acid transporter 1; MM: mouse monoclonal; NRF1: nuclear respiratory factor 1; PGC-1α: peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PPARβ/δ: peroxisome proliferator-activated receptor beta/delta; PPARγ: peroxisome proliferator-activated receptor gamma; SREBP1: sterol regulatory element-binding proteins; RP: rabbit polyclonal; TFAM: mitochondrial transcription factor A; and UCP: uncoupling protein 1/2/3. Notes: Target molecular weight was based on product datasheet. Molecular weights for all targets were verified against sizes suggested by product brochures.