Peroxisome Proliferator-Activated Receptor γ Regulates Lipid Metabolism in Sheep Trophoblast Cells through mTOR Pathway-Mediated Autophagy

Figure 1

Effects of activation and inhibition of PPARγ activity on autophagy-related proteins and lipid metabolism in trophoblast cells. (a) After activating and inhibiting PPARγ activity by rosiglitazone (RSG) and GW9662 (GW), respectively, Western blot was used to detect the levels of p-p70S6K, p70S6K, Beclin1, LC3B-II, and LC3B-I proteins at 12 h, 24 h, and 48 h. (b) TG content at different timepoints. (c) MDA content as an indicator of lipid oxidation at different timepoints. Data are shown as of three independent trials. and .

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