Peroxisome Proliferator-Activated Receptor γ Regulates Lipid Metabolism in Sheep Trophoblast Cells through mTOR Pathway-Mediated Autophagy

Figure 3

Effect of autophagy induced by mTOR on PPARγ regulation of lipid metabolism in STCs. (a) The levels of p-p70S6K, p70S6K, Beclin1, LC3B-II, and LC3B-I proteins at 12 h, 24 h, and 48 h were detected by Western blot after inhibiting PPARγ activity before blocking mTOR pathway and after inhibiting autophagy before activating PPARγ activity. (b) TG content at different timepoints. (c) MDA content as an indicator of lipid oxidation at different timepoints. Data are shown as of three independent trials. , , ^A, and ^ns.

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