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Pain Research and Management
Volume 2, Issue 1, Pages 15-18
Original Article

Amino Acid Levelsin the Brain of Rats with Peripheral Nerve Constriction or Section

Mauro Bianchi, Paolo Ferrario, Antonio Clavenna, Gabriele Biella, and Maria Luisa Sotgiu

Department of Pharmacology, University of Milano, and Institute of Neuroscience and Bioimaging, CNR, Milano, Italy

Copyright © 1997 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


BACKGROUND: Both excitatory and inhibitory amino acids modulate central neuronal activity following peripheral nerve tissue injury.

OBJECTIVE: To establish whether mononeuropathy induced by chronic constriction injury or by sectioning of the sciatic nerve affects neurotransmitter amino acid levels in the rat brain areas.

METHODS: Glutamine, glutamic acid, gamma-aminobutyric acid (GABA) and aspartic acid in the hindbrain, midbrain and hypothalamus of rats were measured by high performance liquid chromatography 14 days after surgery.

RESULTS: After monolateral nerve ligation an increase in glutamine and a decrease in GABA levels in all brain areas were observed, with no side preference. The section of the sciatic nerve did not cause any modification in amino acid levels.

CONCLUSIONS: The results allow one to differentiate more clearly classic models of mononeuropathy from a biochemical point of view. Moreover, results indicate that continuous neuronal stimulation induced by nerve constriction is able to modify some amino acid pathways at a supraspinal level.