Preconditioning Contractions Suppress Muscle Pain Markers after Damaging Eccentric Contractions

Table 1

Real-time RT PCR primer sequences.

Gene	Accession number		Sequence	Primer position
GAPDH	NM_017008.4	Forward	GCTCTCTGCTCCTCCCTGTTC	4–24
		Reverse	GAGGCTGGCACTGCACAA	44–61
MHC-embryonic	NM_012604.1	Forward	CTTCAAACTGAAAAACGCCTATGA	4536–4559
		Reverse	GTTCTAAGTTCTTATTCTCTCGCTTCACA	4584–4612
MHC-neonatal	NM_001100485.1	Forward	ATCAGTGCCAATCCCTTGCT	742–761
		Reverse	CCAAAGCGAGGAGTTGTCA	795–815
HGF	NM_017017.2	Forward	AAAACTACATGGGCAACTTATCCAA	1335–1359
		Reverse	ATGACGGTGTAAATCCTCCATATTC	1396–1420
Pax7	NM_001191984.1	Forward	AAAAGATTGAGGAGTATAAGAGGGAGAA	347–374
		Reverse	GCCGGTCCCGGATTTC	394–409
MyoD	NM_176079.1	Forward	GACGGCTCTCTCTGCTCCTTT	259–279
		Reverse	AGTCGAAACACGGATCATCATAGA	296–319
Myogenin	M24393.1	Forward	GACCCTACAGGTGCCCACAA	604–623
		Reverse	CCGTGATGCTGTCCACGAT	643–661
BKB2 receptor	M59967.2	Forward	GAGCTTGAAGCATCCTAGGGAAT	1543–1565
		Reverse	CGCTTATGCCGTGAGACAAGA	1583–1603
COX-2	U03389.1	Forward	GGCAAAGGCCTCCATTGAC	1423–1441
		Reverse	GCGTTTGCGGTACTCATTGA	1470–1489
mPGES-1	NM_021583.3	Forward	TGCTCCCCGCCTTTCTG	78–94
		Reverse	CCACCGCGTACATCTTGATG	115–134

GAPDH, glyceraldehydes-3-phosphate dehydrogenase; MHC, myosin heavy chain; HGF, hepatocyte growth factor; Pax7, paired box transcription factor-7; MyoD, myogenic determination factor; BKB₂, bradykinin B₂ receptor; COX-2, cyclooxygenase 2; mPGES-1, microsomal prostaglandin E synthase-1.