Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo

Figure 4

Effects of WSLP/siRNA intrathecal injection on rats with inflammatory pain. Rats were measured the MWT and TWL 2 days before and 3, 4, and 5 days after intrathecal injection. (a) A detailed timeline of animal study during scoring MWT and TWL. (b) The MWT of rats was significantly reduced after the intraplantar injection of CFA and can be effectively increased by intrathecal injection of WSLP/NR2B-siRNA. (c) The TWL of rats also showed a significant decrease after the intrathecal injection of CFA, and intrathecal injection of WSLP/NR2B-siRNA can effectively increase the TWL compared with that in the CFA group. NS: no treatment received; CFA: intraplantar injection of complete Freund’s adjuvant; PEI: intrathecal injection of PEI/NR2B-siRNA; WSLP: intrathecal injection of WSLP/NR2B-siRNA; sWSLP: intrathecal injection of scrambled siRNA; MWT: paw mechanical withdrawal threshold; TWL: paw thermal withdrawal latency. versus CFA group.

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