Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo

Table 1

Expression of NR2B mRNA and protein levels in transfection groups.

	Negative transfection group^a	Control transfection group^b	WSLP transfection group^c
mRNA level^d	0.69 ± 0.18	0.64 ± 0.13	0.35 ± 0.21
Protein level^e	4.36 ± 1.02	4.32 ± 1.09	1.96 ± 0.48

versus the negative transfection group; ^aPC12 cells transfected with NR2B-siRNA; ^bPC12 cells transfected with WSLP/scRNA; ^cPC12 cells transfected with WSLP/NR2B-siRNA; ^dthe mRNA level was calculated by the ratio of NR2B mRNA to β-actin mRNA; ^ethe protein level was calculated as the product of the absorbance and area (OD × mm²). All values represent the mean ± standard deviation.