Antiallodynic Effects of Cannabinoid Receptor 2 (CB₂R) Agonists on Retrovirus Infection-Induced Neuropathic Pain

Figure 5

Effects of CB₂R agonist treatment on macrophage infiltration into the LSC of LP-BM5-infected animals. After LP-BM5 infection, synthetic CB₂R ligands were injected (5 mg/kg, i.p.) twice/wk starting at 5 or 6 wk (HU308) p.i. At 12 wk p.i., LSCs were dissected and infiltrating leukocytes were banded on a 30–70% Percoll cushion, collected, and labeled with Abs specific for CD45, CD11b, and MHCII for analysis by flow cytometry. (a) Representative contour plots show the percentages of macrophages (i.e., CD45^hiCD11b^hi) expressing MHCII within uninfected (UI), infected (IF), and infected (IF + indicated CB₂R ligand), agonist-treated LSC. (b) Numbers of macrophages infiltrating into the LSC of animals belonging to the indicated treatment groups. (c) Frequency of macrophages expressing MHCII within the LSC of animals belonging to the indicated groups. Pooled data present absolute numbers (mean ± SE) from two independent experiments using 4–6 animals/group. ; .

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