Skin/Muscle Incision and Retraction Induces Evoked and Spontaneous Pain in Mice

Figure 5

The application of gabapentin systemically raised the paw withdrawal threshold (PWT) in skin/muscle incision and retraction (SMIR) mice. (a) Saline had no effect on the PWTs in the sham and SMIR groups injected at day 4 after SMIR (two-way RM ANOVA, sham versus SMIR: F1; 17 = 35.83, ; treatments: F1; 17 = 0.55, , interaction: F1; 17 = 1.74, , n = 7 for the sham group; n = 8 for the SMIR group, under Sidak’s multiple comparisons test). (b) SMIR decreased the PWTs, which were not changed by the application of gabapentin at 10 mg/kg (two-way RM ANOVA, sham versus SMIR: F1; 17 = 71.68, ; treatments: F1; 17 = 0.25, , interaction: F1; 17 = 0.19, ; n = 9 for the sham group, n = 10 for the SMIR group, under Sidak’s multiple comparisons test). (c) Gabapentin at 20 mg/kg increased the PWTs of the SMIR group (two-way RM ANOVA, sham versus SMIR: F1; 17 = 13.41, ; treatments: F1; 17 = 57.53, , interaction: F1; 17 = 36.69, ; n = 9 for the sham group, n = 10 for the SMIR group, under Sidak’s multiple comparisons test). (d) Gabapentin at 50 mg/kg increased the PWTs of both the SMIR group and sham group (two-way RM ANOVA, sham versus SMIR: F1; 9 = 0.73, ; treatments: F1; 9 = 102.7, , interaction: F1; 9 = 19.41, ; n = 5 for the sham group, n = 6 for the SMIR group, under Sidak’s multiple comparisons test). ANOVA, analysis of variance; RM, repeated measures.

(a)

(b)

(c)

(d)