Locating the Site of Neuropathic Pain In Vivo Using MMP-12-Targeted Magnetic Nanoparticles

Figure 4

Demyelination and elastin fibre loss occurred at the site of ligation. (a–d) Representative images of Luxol Fast Blue (myelin: blue) and Verhoeff-van Gieson (elastin: black, nuclei: blue, and collagen: red) staining, on consecutive sections of the left L5 spinal nerve distal to the DRG, 1 week after SNL or sham surgery (scale bar = 100 μm). (e) Myelin- and (f) elastin-positive areas of SNL and sham rats’ spinal nerves were quantified ( per group). Statistically significant difference in the left L5 spinal nerve myelin content was determined by Mann–Whitney U test ().