Hong-Hui-Xiang Alleviates Pain Hypersensitivity in a Mouse Model of Monoarthritis

Figure 3

The anti-inflammatory effect of HHX in a mouse model of monoarthritis. (a) Histological changes of synovitis were observed in H&E-stained sections. Each knee joint shown was a representative for a group of mice; scale bars represents 200 μm. (b) The synovitis scores for each group from the H&E-stained sections. Synovitis scores in CFA increased significantly (t₈ = 9.391, ) and decreased in HHX at doses of 10 μl and 50 μl (t₈ = 5.715, ; t₈ = 4.914, ), but not at the dose of 1 μl (t₈ = 1.134, ) at day 26. ((c)–(e)) The effect of HHX (1, 10, and 50 μl) on knee joint thickness. CFA exhibited a significant increase in knee joint thickness (time × group interaction, F_{(4, 40)} = 98.74, ). Time course of HHX at doses of 10 μl and 50 μl decreased the knee joint diameter (time × group interaction, F_{(4, 40)} = 20.52, ; F_{(4, 40)} = 14.45, ). However, HHX at doses of 1 μl did not decrease the knee joint diameter (time × group interaction, F_{(4, 40)} = 1.597, ). versus CFA + saline.

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