Comparative Study of Spermatogenesis and Nucleolar Behavior in Testicular Lobes of Euschistus heros (Heteroptera: Pentatomidae)
Figure 3
Spermiogenesis cells stained with lacto-acetic orcein ((a)–(h)) and silver impregnated ((i)–(t)). (a)–(d) Development of spermatids in lobes 1–3 and 5. (a) Round spermatids showing heteropyknotic material at the periphery and center of the nucleus (arrows) and a vesicle in the posterior region (arrowhead); (b) spermatid in elongation with heteropyknotic material at the periphery of the nucleus (arrow); (c) in a following stage, the heteropyknotic chromatin is visualized on only one side (arrow); (d) in a later stage, it becomes indistinguishable; (e)–(h) spermatids of lobes 4 and 6; (e) round spermatids showing a vesicle in the posterior region (arrowhead); (f) spermatid in elongation; (g) spermatid at the later stage of development with chromatin stained weakly and in the posterior region with a protuberance (arrow); (h) sperm showing a thin heteropyknotic chromatin on one side of the head (arrow); ((i)–(m)) round spermatid with several silver-positive bodies; ((j)–(l)) during the subsequent phases were observed three silver-positive bodies: one rod-shape and lighter close to region of tail formation ((j), smaller arrow), one round intensely stained (arrowhead), both in the posterior region of the spermatid and another smaller than others (bigger arrow) that was visualized in the middle region of the spermatid ((k), (l)); (m) spermatid in development with only one silver-positive body in the posterior region of the nucleus (arrow); (n) spermatozoon of lobes 1–3 with continuous silver-positive staining in the posterior region of the head (arrow) and in lobe 5 (o) a continuous positive silver impregnation (arrow) and several small bodies in all the nucleus (arrowhead). ((p)–(t)) Spermiogenesis of lobes 4 and 6; (p) spermatid in development showing strong silver-positive body in the posterior region of the head (arrow), several small silver-positive bodies in the middle region and a line-shaped silver staining from middle to anterior region (arrowhead); ((q)–(t)) developed spermatids showing four silver-positive behaviors: (q) continuous and linear (arrow); (r) large and amorphous; (s) linear and throughout the extent of the head and several round and small silver-positive bodies and (t) with the same previous characteristics, but the silver-positive bodies are larger and smaller in number. Bar= 10 m
