Induction of Manduca sexta Larvae Caspases Expression in Midgut Cells by Bacillus thuringiensis Cry1Ab Toxin

Figure 3

Representative electrophoretic patterns of RT-PCR amplifications of cas-1, cas-3, and rpS3 genes from M. sexta larvae. RT-PCR analysis of the expression of cas-1, cas-3, and rpS3 genes at different times were performed with control larvae or with Cry1Ab intoxicated M. sexta larvae with two different concentrations of Cry1Ab toxin. The numbers on the agarose gel represent the number of pixels observed in each mRNA band as determine by scanning optical density of the bands.