Wild Bumblebee (Bombus) Diversity and Nosema (Microsporidia: Nosematidae) Infection Levels Associated with Lowbush Blueberry (Vaccinium angustifolium) Production and Commercial Bumblebee Pollinators

Table 2

Primers used for PCR amplification of ribosomal RNA.

Name	Strand direction	Sequence (5′-3′)	Annealing temp. (C)	Expected fragment size
SSUrRNA-fl	Forward	CACCAGGTTG
		ATTCTGCCT	48	222
SSUrRNA-rlc	Reverse	GTTACCCGTC
		ACTGCCTTG
Nbombi-SSU-Jfl	Forward	CCATGCATGTT
		TTTGAAGATTATTAT	50	323
Nbombi-SSU-Jrl	Reverse	CATATATTTTTA
		AAATATGAAACAATAA

Based on Klee et al., 2006 [28], developed from the complete rRNA N. bombi consensus sequence, Accession no. AY741110.