Table 2: Primers used for PCR amplification of ribosomal RNA.

 Name Strand directionSequence (5′-3′)Annealing temp. (C)Expected fragment size

SSUrRNA-flForwardCACCAGGTTG
ATTCTGCCT48222
SSUrRNA-rlcReverseGTTACCCGTC
ACTGCCTTG

Nbombi-SSU-JflForward CCATGCATGTT
TTTGAAGATTATTAT50323
Nbombi-SSU-JrlReverseCATATATTTTTA
AAATATGAAACAATAA

Based on Klee et al., 2006 [28], developed from the complete rRNA N. bombi consensus sequence, Accession no. AY741110.