Ovarian Development and Vitellogenin Gene Expression under Heat Stress in Silkworm, Bombyx mori

Figure 1

Effect of heat stress on gonadosomatic index (ovary). Heat shock was given to pupae, 2 days after larval-pupal ecdysis and developing ovarioles were dissected out from pupae on days 4, 6, and 8 from each experimental group. The wet weight of developing ovaries was measured after rinsing with insect ringer solution and gonadosomatic index was calculated with respect to total body weight. Each histogram bar represents the mean gonadosomatic index (–8) and the error bar represents standard error of the mean (SEM). The asterisks show the data which are significantly different from control sample (unpaired -test; , , and ). NS: not significant at . C: control; 39-1: heat shock at 39°C for 1 h; 39-2: heat shock at 39°C for 2 h; 42-1: heat shock at 42°C for 1 h; 42-2: heat shock at 42°C for 2 h; 45-1: heat shock at 45°C for 1 h; 45-2: heat shock at 45°C for 2 h.