Differential Effects of Alpha-Particle Radiation and X-Irradiation on Genes Associated with Apoptosis

Table 2

(a) Genes responsive at all doses. Statistically significant differentially expressed genes with corresponding P values and fold changes (FC) which were found to respond at all doses of α-particle radiation tested (0.5, 1.0, and 1.5 Gy) and were harvested 4 days after exposure listed by descending FC. Statistical cut-off at 𝑃≤.05, with 𝑛=3 biological replicates. (b) Genes responsive at medium and high doses. Statistically significant differentially expressed genes with corresponding 𝑃values and fold changes (FC) which were found to respond at 1.0 Gy and 1.5 Gy of α-particle radiation harvested 4 days after exposure listed by descending FC. Statistical cut-off at 𝑃≤.05, with 𝑛=3 biological replicates. (c) Genes responsive at high doses. Statistically significant differentially expressed genes with corresponding 𝑃values and fold changes (FC) which were found to respond at 1.5 Gy of α-particle radiation and were harvested 4 days after exposure listed by descending FC. Statistical cut-off at 𝑃≤.05, with 𝑛=3 biological replicates.

(a)

Accession #	Gene name	0.5 Gy		1.0 Gy		1.5 Gy
		FC	𝑃 Value	FC	𝑃 Value	FC	𝑃 Value
NM_000594	TNF	4.68	.00	5.43	.00	4.25	.00
NM_004347	CASP5	1.65	.04	2.78	.01	2.93	.00
NM_001561	TNFRSF9	2.82	.00	2.65	.00	2.87	.00
NM_000043	FAS	1.82	.00	1.75	.00	1.77	.01
NM_021960	MCL1	1.54	.00	1.59	.02	1.25	.02
NM_003879	CFLAR	−1.27	.02	−1.51	.01	−1.33	.01
NM_004323	BAG1	−1.21	.01	−1.41	.00	−1.39	.00
NM_001166	BIRC2	−1.49	.04	−1.44	.04	−1.46	.02
NM_012423	RPL13A	−1.30	.04	−1.38	.03	−1.64	.00
NM_014739	BCLAF1	−1.49	.01	−1.90	.00	−1.68	.00
NM_003805	CRADD	−1.36	.02	−1.51	.00	−1.72	.00
NM_001924	GADD45A	−1.79	.03	−1.90	.03	−1.72	.01
NM_000633	BCL2	−1.59	.00	−2.13	.00	−2.07	.00
NM_001065	TNFRSF1A	−2.31	.02	−2.51	.03	−2.11	.03
NM_000875	IGF1R	−1.92	.01	−1.77	.01	−2.21	.00

(b)

Accession #	Gene name	1.0 Gy		1.5 Gy
		FC	𝑃 Value	FC	𝑃 Value
NM_004049	BCL2A1	1.87	.00	2.03	.00
NM_004048	B2M	1.27	.02	1.34	.00
NM_001196	BID	−1.31	.01	−1.39	.00
NM_001229	CASP9	−1.38	.01	−1.39	.04
NM_000546	TP53	−1.38	.02	−1.43	.01
NM_014959	CARD8	−1.69	.01	−1.72	.00
NM_000074	CD40LG	−2.82	.03	−2.54	.03

(c)

Accession #	Gene name	1.5 Gy
		FC	𝑃 value
NM_004295	TRAF4	1.94	.02
NM_004281	BAG3	1.28	.02
NM_003842	TNFRSF10B	−1.24	.02
NM_003921	BCL10	−1.30	.04
NM_004050	BCL2L2	−1.53	.00