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Volume 2017, Article ID 5067867, 8 pages
Research Article

Effect of Red Light-Emitting Diodes Irradiation on Hemoglobin for Potential Hypertension Treatment Based on Confocal Micro-Raman Spectroscopy

1MOE Key Laboratory of Laser Life Science & SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology, College of Biophotonics, South China Normal University, Guangzhou, China
2Guzhen Productivity Promotion Center, Zhongshan, China

Correspondence should be addressed to Zhengfei Zhuang; nc.ude.uncs@fzgnauhz and Zhouyi Guo; nc.ude.uncs@nna

Received 19 July 2016; Revised 17 October 2016; Accepted 23 October 2016; Published 12 January 2017

Academic Editor: Alessio Morelli

Copyright © 2017 Xuejun Qiu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Red light-emitting diodes (LED) were used to irradiate the isolated hypertension hemoglobin (Hb) and Raman spectra difference was recorded using confocal micro-Raman spectroscopy. Differences were observed between the controlled and irradiated Hb by comparing the spectra records. The Raman spectrum at the 1399 cm−1 band decreased following prolonged LED irradiation. The intensity of the 1639 cm−1 band decreased dramatically in the first five minutes and then gradually increased in a time-dependent manner. This observation indicated that LED irradiation increased the ability of oxygen binding in Hb. The appearance of the heme aggregation band at 1399 cm−1, in addition to the oxygen marker band at 1639 cm−1, indicated that, in our study, 30 min of irradiation with 15.0 mW was suitable for inhibiting heme aggregation and enhancing the oxygen-carrying capacity of Hb. Principal component analysis showed a one-to-one relationship between irradiated Hb at different time points and the corresponding Raman spectra. Our approach could be used to analyze the hemoglobin from patients with confocal micro-Raman spectroscopy and is helpful for developing new nondrug hypertension therapy.