Cytotoxicity of Etch-and-Rinse, Self-Etch, and Universal Dental Adhesive Systems in Fibroblast Cell Line 3T3

Figure 3

Micrographs from SEM analyses of 3T3 fibroblasts. (a) SBU, no changes in morphology compared with those in the control group, ×1000; (b) SBU, abundant cytoplasm with numerous elongated thin cytoplasmic projections adhering to the glass surface, ×500; (c) AMU, similar morphology to the control group, ×1000; (d) AMU, long, thin cytoplasmic membrane derived prolongations, ×500; (e) OPT, altered morphology with ill-defined cellular limits, suggesting cell necrosis, ×1000; (f) OPT, morphological changes and ruptured membranes in 3T3 cells, ×500.

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