Identifying Two Common Types of Breast Benign Diseases Based on Multiphoton Microscopy

Table 2

MPM diagnostic features of normal breast tissue, fibroadenoma lesion, and fibrocystic disease.

Breast tissues	MPM diagnostic features
Normal breast tissue	(1) Epithelial cells inside acinus generate strong TPEF signal, showing the regular arrangement and uniform cell size; (2) Collagen fibers inside lobule emit strong SHG signal, exhibiting circular morphological feature
Fibroadenoma lesion	(1) Mammary ducts are distinctly compressed; (2) Collagen fiber increases in density relatively, appears less organized, and becomes shorter
Fibrocystic disease	(1) The cysts are visualized with lumen filled with cystic fluid; (2) The collagen fibrous tissues surrounding the cysts are denser compared to normal breast tissue