A Novel Fluorescent Dye Extracted from Buddleja officinalis for Labeling Mitochondria after Fixation

Figure 2

Costaining of phalloidin, 4,6-diamidino-2-phenylindole (DAPI), and BO-dye in fixed immortalized human oral keratinocytes (IHOKs). (a) Phalloidin channel. (b) DAPI channel. (c) BO-dye channel. (d) Merged channel. BO-dye was distributed around the nucleus but not inside the nucleus and cytomembrane (white arrow). Scale bar is set to 100 μm.