[Retracted] Clinical Analysis of Echocardiography and Serum IL-6 and TNF-α Changes in Pregnant Women with Hypertension

Table 2

Components added after brief centrifugation.

Reagent	Volume
M-MLV RT 5 × reaction buffer	5 μl
dATP, 10 mM	1.25 μl
dCTP, 10 mM	1.25 μl
dGTT, 10 mM	1.25 μl
dTTP, 10 mM	1.25 μl
M-MLV RT (H-) point mutant	1 μl (50–100 units)
Nuclease-free water replenishes the total volume	25 μl