A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech
Figure 2
Differential expression of Tpr during leech embryogenesis. (a) Differential display analysis shows faint Tpr bands (boxed with pinholes) in teloblast lanes M and N, and in stage 7 embryos which contain teloblasts M, N, O, P and Q. Bands above and below appear in all lanes and are likely “housekeeping” genes. (b) Semi-quantitative RT-PCR analysis shows declining Tpr transcripts during stage 1 (presumably maternal) through stage 4; accumulation of new zygotic transcripts was evident thereafter and peaked at stage 7. Lanes were normalized with 18S rRNA primers.
