Culture and Use of Mesenchymal Stromal Cells in Phase I and II Clinical Trials
Table 1
Features of bone-marrow derived mesenchymal stem cells (MSCs) and adipose-derived stem cells (ADSCs).
Feature
MSCs
ADSCs
Tissue sampling
Adult
Adult
General anesthesia
Local anesthesia
Cell purification process
No proteolytic digestion
Proteolytic digestion, generate different cell subsets
Phenotype
CD49a, CD73, CD90, CD105 CD271 and negative for CD34
CD49a, CD73, CD90, CD105 (CD271?) and CD34 in early passage
Frequency (CFU-Fs)
0.005
0.05
Potency
Hematopoietic support Classic mesenchymal lineage (adipo-, chondro- and osteogenesis) Immunosuppressive properties Precommitted towards osteogenesis (cultured MSCs)
Hematopoietic support Classic mesenchymal lineage (adipo-, chondro- and osteogenesis) Immunosuppressive properties Precommitted towards adipogenesis Good angiogenic potential
