Primitive Sca-1 Positive Bone Marrow HSC in Mouse Model of Aplastic Anemia: A Comparative Study through Flowcytometric Analysis and Scanning Electron Microscopy

Figure 1

(a) Gated region of flowcytometric analysis. (b) Phenotypic characterization of Sca-1 expression by normal bone marrow cells. R-1 region indicates the $\text{Sca-}{1}^{\text{dim}}$ population (8.83%). R-2 region indicates the $\text{Sca-}{1}^{\text{bright}}$ population (12.23%). (c) Phenotypic characterization of Sca-1 expression by Aplastic anemic bone marrow cells. R-2 region, that is, $\text{Sca-}{1}^{\text{bright}}$ population is more severely affected (0.01%) compared to the $\text{Sca-}{1}^{\text{dim}}$ population in the R-1 region.

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