Plasticity of Cells and Ex Vivo Production of Red Blood Cells

Figure 1

Culture protocol for the efficient production of enucleated red blood cells without feeder cells from hematopoietic stem/progenitor cells. Passage I~III are the steps to expand erythroid progenitor cells. Passage IV is the step to induce enucleation of progenitor cells. SCF, stem cell factor; EPO, erythropoietin; IL-3, interleukin-3. VEGF, vascular endothelial growth factor; IGF-II, insulin-like growth factor-II; MAP, mixture of D-mannitol, adenine, and disodium hydrogen phosphate dodecahydrate.