Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential
Figure 6
Effect of culture conditions on the immunosuppressive activity of hMSCs. Immunosuppressive activity of medium conditioned by IL-1β-stimulated hMSCs expanded in control or FGF-supplemented medium. Values are normalized to the inhibitory activity of first passage hMSCs expanded in control conditions. (a) Immunosuppressive activity (mean ± standard deviation; ) of medium conditioned by IL-1β-stimulated hMSCs from different passages expanded in control (black bars) or FGF-supplemented (white bars) medium. (b) Immunosuppressive activity of medium conditioned by IL-1β-stimulated hMSCs expanded in control (black circles) or FGF-supplemented (white circles) medium. The data are presented as pairs of preparation- and passage-matched hMSCs subsets. Note that for 8 of the 11 data pairs (73%) the conditioned medium from FGF-treated hMSCs is more immunosuppressive than that of their control counterparts. Overall, the differences in immunosuppressive activity between conditioned media from control and FGF-treated cells are significant (one-tail paired t-test; ).
