Enhanced Healing of Diabetic Wounds by Topical Administration of Adipose Tissue-Derived Stromal Cells Overexpressing Stromal-Derived Factor-1: Biodistribution and Engraftment Analysis by Bioluminescent Imaging
Figure 2
In vivo angiogenesis gel plug assay. AT-SCs isolated from ubiquitously GFP-expressing mice were mixed with Cultrex and injected subcutaneously into CD1 recipient. After 1 week, gel plugs were analyzed and capillary density was determined by Masson Trichromic stain (b) and anti-GFP immunohistochemistry (d). Control animals received gel plugs containing saline solution (a)–(c). Masson trichromic stain of implanted gel plugs containing nonconditioned serum-free medium (e) and conditioned medium from AT-SCs cultures (f); AT-SCs were genetically modified by Ad-GFP (g) and Ad-SDF-1 (h). The number of endothelial-like structures formation was slightly improved by SDF-1 overexpression, but the difference versus the control group (Ad-GFP) did not achieve statistical significance (-value ).
