Enhanced Healing of Diabetic Wounds by Topical Administration of Adipose Tissue-Derived Stromal Cells Overexpressing Stromal-Derived Factor-1: Biodistribution and Engraftment Analysis by Bioluminescent Imaging
Figure 3
Wound closure in diabetic animals. Effect of topical administration of AT-SCs at the lesion site. Four groups of wounded diabetic mice were treated with saline solution (Saline), AT-SCs not genetically modified (AT-SCs) or AT-SCs after adenoviral-mediated gene transfer of GFP (AT-SCs GFP), and SDF-1 (AT-SCs SDF-1). At different time points after wounding, the area of the lesion was determined using image analysis. Re-epithelialization is reported as the percentage of the initial wound area (a). Representative images from an animal from each group were taken immediately after wounding (day 0) and at different time points after injury (b). Data are expressed as mean ± SEM indicated by error bars; n = 8–12 wounds for each group. Values of the AT-SCs, AT-SCs GFP, and AT-SCs SDF-1 were significantly different () when compared to the saline group at the time points indicated by an asterisk. Moreover, at 3 and 5 days, wound closure was improved in AT-SCs SDF-1-treated animals in comparison with the AT-SCs GFP group ().
