Enhanced Healing of Diabetic Wounds by Topical Administration of Adipose Tissue-Derived Stromal Cells Overexpressing Stromal-Derived Factor-1: Biodistribution and Engraftment Analysis by Bioluminescent Imaging
Figure 5
Ex vivo and in vivo imaging of AT-SCs expressing luciferase at time 0. Freshly isolated AT-SCs from luciferase-expressing mice were subjected to adenoviral-mediated gene transfer ((a) non-transduced; (b) Ad-mock-transduced; (c) SDF-1-transduced cells). The AT-SCs were placed in a 96-well cell culture plate and BLI imaging was performed (insert of each figure panel). The same cells were then administered to hyperglycemic mice right after performing a dorsal lesion and BLI was repeated. Panel (d) shows quantification of the signals. Note. The diameter of a well of a 96-well plate is 6.4 mm; the diameter of the biopsy punch used to induce wounding is 3.5 mm. Data are expressed as mean ± SEM indicated by error bars; for each group.
