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Stem Cells International
Volume 2011, Article ID 368192, 9 pages
http://dx.doi.org/10.4061/2011/368192
Research Article

Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells

1Centre National de la Recherche Scientifique, Institut André Lwoff, 7, Rue Guy Moquet, 94800 Villejuif, France
2CNRS, Institut de Chimie des Substances Naturelles (ICSN), Avenue de la Terrasse, 91190 Gif Sur Yvette, France
3CNRS, Institut de Génétique Humaine (IGH), 141, Rue Cardonille, 34396 Montpellier, France
4Institut National de la Santé et Recherche Médicale U972, and University Paris 11, Hôpital de Bicêtre, 80 Avenue du Général Leclerc, 94276 Kremlin Bicêtre Cedex, France

Received 4 April 2011; Accepted 22 May 2011

Academic Editor: Jackie R. Bickenbach

Copyright © 2011 Romain Barbet et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We present a strategy to identify developmental/differentiation and plasma membrane marker genes of the most primitive human Mesenchymal Stem Cells (hMSCs). Using sensitive and quantitative TaqMan Low Density Arrays (TLDA) methodology, we compared the expression of 381 genes in human Embryonic Stem Cells (hESCs), hESC-derived MSCs (hES-MSCs), and hMSCs. Analysis of differentiation genes indicated that hES-MSCs express the sarcomeric muscle lineage in addition to the classical mesenchymal lineages, suggesting they are more primitive than hMSCs. Transcript analysis of membrane antigens suggests that , , , , and CD34 may be good candidates for the detection and isolation of the most primitive hMSCs. The expression in hMSCs of cytokine genes, such as IL6, IL8, or FLT3LG, without expression of the corresponding receptor, suggests a role for these cytokines in the paracrine control of stem cell niches. Our database may be shared with other laboratories in order to explore the considerable clinical potential of hES-MSCs, which appear to represent an intermediate developmental stage between hESCs and hMSCs.