The Role of Glucose, Serum, and Three-Dimensional Cell Culture on the Metabolism of Bone Marrow-Derived Mesenchymal Stem Cells
Figure 3
Fluorescence microscopy images indicating the viability of BMSCs in two-dimensional culture after the second day (increasing glucose concentrations from left to right; increasing serum concentrations from top to bottom). Calcein-AM indicates living cells by emitting green light, while ethidium bromide indicates dead cells by emitting red light. The 0.5 mM and 1.0 mM environments resulted in low viability and sparse cell distribution. The 5.0 mM and 25.0 mM environments resulted in high viability and the maintenance of high confluency. Varying the serum concentration did not produce a noticeable difference in the viability. This result was consistent throughout the course of the week (20x objective; scale bar represents 250 μm).
