Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2011 (2011), Article ID 429187, 12 pages
http://dx.doi.org/10.4061/2011/429187
Research Article

The Role of Glucose, Serum, and Three-Dimensional Cell Culture on the Metabolism of Bone Marrow-Derived Mesenchymal Stem Cells

1Weldon School of Biomedical Engineering, Purdue University, 585 Purdue Mall, West Lafayette, IN 47907-2088, USA
2School of Mechanical Engineering, Purdue University, 585 Purdue Mall, West Lafayette, IN 47907-2088, USA
3Department of Basic Medical Sciences, Purdue University, 585 Purdue Mall, West Lafayette, IN 47907-2088, USA

Received 3 December 2010; Accepted 7 February 2011

Academic Editor: Kenneth R. Boheler

Copyright © 2011 Byron Deorosan and Eric A. Nauman. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. D. Metcalfe and M. W. J. Ferguson, “Tissue engineering of replacement skin: the crossroads of biomaterials, wound healing, embryonic development, stem cells and regeneration,” Journal of the Royal Society Interface, vol. 4, no. 14, pp. 413–417, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  2. A. D. Metcalfe and M. W. J. Ferguson, “Bioengineering skin using mechanisms of regeneration and repair,” Biomaterials, vol. 28, no. 34, pp. 5100–5113, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  3. S. Llames, E. García, V. García et al., “Clinical results of an autologous engineered skin,” Cell and Tissue Banking, vol. 7, no. 1, pp. 47–53, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  4. F. Opitz, K. Schenke-Layland, T. U. Cohnert, and U. A. Stock, “Phenotypical plasticity of vascular smooth muscle cells—effect of in vitro and in vivo shear stress for tissue engineering of blood vessels,” Tissue Engineering, vol. 13, no. 10, pp. 2505–2514, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  5. B. M. Leung and M. V. Sefton, “A modular tissue engineering construct containing smooth muscle cells and endothelial cells,” Annals of Biomedical Engineering, vol. 35, no. 12, pp. 2039–2049, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  6. J. Y. Liu, D. D. Swartz, H. F. Peng, S. F. Gugino, J. A. Russell, and S. T. Andreadis, “Functional tissue-engineered blood vessels from bone marrow progenitor cells,” Cardiovascular Research, vol. 75, no. 3, pp. 618–628, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  7. G. E. Amiel, M. Komura, OZ. Shapira et al., “Engineering of blood vessels from acellular collagen matrices coated with human endothelial cells,” Tissue Engineering, vol. 12, no. 8, pp. 2355–2365, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  8. S. Heydarkhan-Hagvall, M. Esguerra, G. Helenius, R. Söderberg, B. R. Johansson, and B. Risberg, “Production of extracellular matrix components in tissue-engineered blood vessels,” Tissue Engineering, vol. 12, no. 4, pp. 831–842, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  9. A. N. Morritt, S. K. Bortolotto, R. J. Dilley et al., “Cardiac tissue engineering in an in vivo vascularized chamber,” Circulation, vol. 115, no. 3, pp. 353–360, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  10. X. M. Guo, Y. S. Zhao, H. X. Chang et al., “Creation of engineered cardiac tissue in vitro from mouse embryonic stem cells,” Circulation, vol. 113, no. 18, pp. 2229–2237, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  11. M. Radisic, H. Park, F. Chen et al., “Biomimetic approach to cardiac tissue engineering: oxygen carriers and channeled scaffolds,” Tissue Engineering, vol. 12, no. 8, pp. 2077–2091, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  12. S. Heber, L. Denk, K. Hu, and W. W. Minuth, “Modulating the development of renal tubules growing in serum-free culture medium at an artificial interstitium,” Tissue Engineering, vol. 13, no. 2, pp. 281–292, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  13. W. W. Minuth, L. Denk, and K. Hu, “The role of polyester interstitium and aldosterone during structural development of renal tubules in serum-free medium,” Biomaterials, vol. 28, no. 30, pp. 4418–4428, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  14. D. C. Chen, J. R. Avansino, V. G. Agopian et al., “Comparison of polyester scaffolds for bioengineered intestinal mucosa,” Cells Tissues Organs, vol. 184, no. 3-4, pp. 154–165, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  15. Y. Nakase, A. Hagiwara, T. Nakamura et al., “Tissue engineering of small intestinal tissue using collagen sponge scaffolds seeded with smooth muscle cells,” Tissue Engineering, vol. 12, no. 2, pp. 403–412, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  16. F. Bolland, S. Korossis, S. P. Wilshaw et al., “Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering,” Biomaterials, vol. 28, no. 6, pp. 1061–1070, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  17. M. Pattison, T. J. Webster, J. Leslie, M. Kaefer, and K. M. Haberstroh, “Evaluating the in vitro and in vivo efficacy of nano-structured polymers for bladder tissue replacement applications,” Macromolecular Bioscience, vol. 7, no. 5, pp. 690–700, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  18. C. Danielsson, S. Ruault, A. Basset-Dardare, and P. Frey, “Modified collagen fleece, a scaffold for transplantation of human bladder smooth muscle cells,” Biomaterials, vol. 27, no. 7, pp. 1054–1060, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  19. M. S. Kim, Y. N. Shin, M. H. Cho et al., “Adhesion behavior of human bone marrow stromal cells on differentially wettable polymer surfaces,” Tissue Engineering, vol. 13, no. 8, pp. 2095–2103, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  20. E. H. Chung, M. Gilbert, A. S. Virdi, K. Sena, D. R. Sumner, and K. E. Healy, “Biomimetic artificial ECMs stimulate bone regeneration,” Journal of Biomedical Materials Research. Part A, vol. 79, no. 4, pp. 815–826, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  21. H. Wang, Y. Li, YI. Zuo, J. Li, S. Ma, and L. Cheng, “Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/polyamide composite scaffolds for bone tissue engineering,” Biomaterials, vol. 28, no. 22, pp. 3338–3348, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  22. D. A. Shimko, C. A. Burks, K. C. Dee, and E. A. Nauman, “Comparison of in vitro mineralization by murine embryonic and adult stem cells cultured in an osteogenic medium,” Tissue Engineering, vol. 10, no. 9-10, pp. 1386–1398, 2004. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  23. S. Terai, I. Sakaida, N. Yamamoto et al., “An in vivo model for monitoring trans-differentiation of bone marrow cells into functional hepatocytes,” Journal of Biochemistry, vol. 134, no. 4, pp. 551–558, 2003. View at Publisher · View at Google Scholar · View at Scopus
  24. X. Wang, H. Willenbring, Y. Akkari et al., “Cell fusion is the principal source of bone-marrow-derived hepatocytes,” Nature, vol. 422, no. 6934, pp. 897–901, 2003. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  25. R. A. Faris, T. Konkin, and G. Halpert, “Liver stem cells: a potential source of hepatocytes for the treatment of human liver disease,” Artificial Organs, vol. 25, no. 7, pp. 513–521, 2001. View at Google Scholar · View at Scopus
  26. M. R. Alison, R. Poulsom, R. Jeffery et al., “Cell differentiation—hepatocytes from non-hepatic adult stem cells,” Nature, vol. 406, no. 6793, p. 257, 2000. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  27. N. D. Theise, M. Nimmakayalu, R. Gardner et al., “Liver from bone marrow in humans,” Hepatology, vol. 32, no. 1, pp. 11–16, 2000. View at Google Scholar · View at Scopus
  28. S. Terai, T. Ishikawa, K. Omori et al., “Improved liver function in patients with liver cirrhosis after autologous bone marrow cell infusion therapy,” Stem Cells, vol. 24, no. 10, pp. 2292–2298, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  29. J. C. Voltarelli, C. E. B. Couri, A. B. P. L. Stracieri et al., “Autologous nonmyeloablative hematopoietic stem cell transplantation in newly diagnosed type 1 diabetes mellitus,” Journal of the American Medical Association, vol. 297, no. 14, pp. 1568–1576, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  30. H. Miszta-Lane, M. Mirbolooki, A. M. James Shapiro, and J. R. T. Lakey, “Stem cell sources for clinical islet transplantation in type 1 diabetes: embryonic and adult stem cells,” Medical Hypotheses, vol. 67, no. 4, pp. 909–913, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  31. D. Hess, L. Li, M. Martin et al., “Bone marrow-derived stem cells initiate pancreatic regeneration,” Nature Biotechnology, vol. 21, no. 7, pp. 763–770, 2003. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  32. Y. Hori, I. C. Rulifson, B. C. Tsai, J. J. Heit, J. D. Cahoy, and S. K. Kim, “Growth inhibitors promote differentiation of insulin-producing tissue from embryonic stem cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 25, pp. 16105–16110, 2002. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  33. N. Lumelsky, O. Blondel, P. Laeng, I. Velasco, R. Ravin, and R. McKay, “Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets,” Science, vol. 292, no. 5520, pp. 1389–1394, 2001. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  34. B. Soria, A. Skoudy, and F. Martin, “From stem cells to beta cells: new strategies in cell therapy of diabetes mellitus,” Diabetologia, vol. 44, no. 4, pp. 407–415, 2001. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  35. K. Shimizu, A. Ito, T. Yoshida, Y. Yamada, M. Ueda, and H. Honda, “Bone tissue engineering with human mesenchymal stem cell sheets constructed using magnetite nanoparticles and magnetic force,” Journal of Biomedical Materials Research. Part B, vol. 82, no. 2, pp. 471–480, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  36. H. Hattori, K. Masuoka, M. Sato et al., “Bone formation using human adipose tissue-derived stromal cells and a biodegradable scaffold,” Journal of Biomedical Materials Research. Part B, vol. 76, no. 1, pp. 230–239, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  37. J. R. Mauney, V. Volloch, and D. L. Kaplan, “Role of adult mesenchymal stem cells in bone tissue-engineering applications: current status and future prospects,” Tissue Engineering, vol. 11, no. 5-6, pp. 787–802, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  38. D. Čížková, J. Rosocha, I. Vanický, S. Jergová, and M. Čížek, “Transplants of human mesenchymal stem cells improve functional recovery after spinal cord injury in the rat,” Cellular and Molecular Neurobiology, vol. 26, no. 7-8, pp. 1167–1180, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  39. Y. B. Deng, Q. T. Yuan, X. G. Liu et al., “Functional recovery after rhesus monkey spinal cord injury by transplantation of bone marrow mesenchymal-stem cell-derived neurons,” Chinese Medical Journal, vol. 118, no. 18, pp. 1533–1541, 2005. View at Google Scholar · View at Scopus
  40. K. H. Lee, H. Suh-Kim, J. S. Choi et al., “Human mesenchymal stem cell transplantation promotes functional recovery following acute spinal cord injury in rats,” Acta Neurobiologiae Experimentalis, vol. 67, no. 1, pp. 13–22, 2007. View at Google Scholar · View at Scopus
  41. J. Rohrbough, E. Rushton, E. Woodruff, T. Fergestad, K. Vigneswaran, and K. Broadie, “Presynaptic establishment of the synaptic cleft extracellular matrix is required for post-synaptic differentiation,” Genes and Development, vol. 21, no. 20, pp. 2607–2628, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  42. C. Streuli, “Extracellular matrix remodelling and cellular differentiation,” Current Opinion in Cell Biology, vol. 11, no. 5, pp. 634–640, 1999. View at Publisher · View at Google Scholar · View at Scopus
  43. J. A. Pedersen and M. A. Swartz, “Mechanobiology in the third dimension,” Annals of Biomedical Engineering, vol. 33, no. 11, pp. 1469–1490, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  44. V. Terraciano, N. Hwang, L. Moroni et al., “Differential response of adult and embryonic mesenchymal progenitor cells to mechanical compression in hydrogels,” Stem Cells, vol. 25, no. 11, pp. 2730–2738, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  45. I. Titushkin and M. Cho, “Modulation of cellular mechanics during osteogenic differentiation of human mesenchymal stem cells,” Biophysical Journal, vol. 93, no. 10, pp. 3693–3702, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  46. J. A. Pedersen, F. Boschetti, and M. A. Swartz, “Effects of extracellular fiber architecture on cell membrane shear stress in a 3D fibrous matrix,” Journal of Biomechanics, vol. 40, no. 7, pp. 1484–1492, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  47. L. E. Freed, F. Guilak, X. E. Guo et al., “Advanced tools for tissue engineering: scaffolds, bioreactors, and signaling,” Tissue Engineering, vol. 12, no. 12, pp. 3285–3305, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  48. M. Schindler, A. Nur-E-Kamal, I. Ahmed et al., “Living in three dimensions: 3D nanostructured environments for cell culture and regenerative medicine,” Cell Biochemistry and Biophysics, vol. 45, no. 2, pp. 215–227, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  49. L. J. Smith, J. S. Swaim, C. Yao et al., “Increased osteoblast cell density on nanostructured PLGA-coated nanostructured titanium for orthopedic applications,” International Journal of Nanomedicine, vol. 2, no. 3, pp. 493–499, 2007. View at Google Scholar
  50. E. Gatlik-Landwojtowicz, P. Aanismaa, and A. Seelig, “The rate of P-glycoprotein activation depends on the metabolic state of the cell,” Biochemistry, vol. 43, no. 46, pp. 14840–14851, 2004. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  51. H. D. Vishwasrao, A. A. Heikal, K. A. Kasischke, and W. W. Webb, “Conformational dependence of intracellular NADH on metabolic state revealed by associated fluorescence anisotropy,” Journal of Biological Chemistry, vol. 280, no. 26, pp. 25119–25126, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  52. B. T. Mischen, K. E. Follmar, K. E. Moyer et al., “Metabolic and functional characterization of human adipose-derived stem cells in tissue engineering?” Plastic and Reconstructive Surgery, vol. 122, no. 3, pp. 725–738, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  53. K. E. Follmar, F. C. Decroos, H. L. Prichard, H. T. Wang, D. Erdmann, and K. C. Olbrich, “Effects of glutamine, glucose, and oxygen concentration on the metabolism and proliferation of rabbit adipose-derived stem cells,” Tissue Engineering, vol. 12, no. 12, pp. 3525–3533, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  54. E. A. Sander and E. A. Nauman, “Effects of reduced oxygen and glucose levels on ocular cells in vitro: implications for tissue models,” Cells Tissues Organs, vol. 191, no. 2, pp. 141–151, 2010. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  55. H. A. Horner and J. P. G. Urban, “2001 Volvo award winner in basic science studies: effect of nutrient supply on the viability of cells from the nucleus pulposus of the intervertebral disc,” Spine, vol. 26, no. 23, pp. 2543–2549, 2001. View at Publisher · View at Google Scholar · View at Scopus
  56. S. Han, J. D. Ritzenthaler, B. Wingerd, H. N. Rivera, and J. Roman, “Extracellular matrix fibronectin increases prostaglandin E receptor subtype EP4 in lung carcinoma cells through multiple signaling pathways: the role of AP-2,” Journal of Biological Chemistry, vol. 282, no. 11, pp. 7961–7972, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  57. I. A. Potapova, G. R. Gaudette, P. R. Brink et al., “Mesenchymal stem cells support migration, extracellular matrix invasion, proliferation, and survival of endothelial cells in vitro,” Stem Cells, vol. 25, no. 7, pp. 1761–1768, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  58. C. Toral, M. E. Mendoza-Garrido, E. Azorín et al., “Effect of extracellular matrix on adhesion, viability, actin cytoskeleton and K+ currents of cells expressing human ether à go-go channels,” Life Sciences, vol. 81, no. 3, pp. 255–265, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  59. C. B. Khatiwala, S. R. Peyton, and A. J. Putnam, “Intrinsic mechanical properties of the extracellular matrix affect the behavior of pre-osteoblastic MC3T3-E1 cells,” American Journal of Physiology, vol. 290, no. 6, pp. C1640–C1650, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  60. E. Gentleman, E. A. Nauman, K. C. Dee, and G. A. Livesay, “Short collagen fibers provide control of contraction and permeability in fibroblast-seeded collagen gels,” Tissue Engineering, vol. 10, no. 3-4, pp. 421–427, 2004. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  61. K. E. Lewus and E. A. Nauman, “In vitro characterization of a bone marrow stem cell-seeded collagen gel composite for soft tissue grafts: effects of fiber number and serum concentration,” Tissue Engineering, vol. 11, no. 7-8, pp. 1015–1022, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus