An Inducible Expression System of the Calcium-Activated Potassium
Channel 4 to Study the Differential Impact on Embryonic Stem Cells
Figure 4
Doxycycline treatment alters cell behavior. (a) Elongated dox treatment for 3 days alters the pluripotency and morphology of the ES cells. Oct3/4 proteins (red) are decreased, and green fluorescence is solely expressed by dox-treated cells. (b) In line, the ES cell marker SSEA1 is lost during dox treatment (red), and the actin cytoskeleton, visualized by the f-actin marker phalloidin, is altered upon dox treatment. (c) Proliferation of iSK4 cells is not changed by dox-treatment shown in a proliferation assay. () for all experiments. Nuclei are stained by DAPI (blue). Scale bars as indicated.
