Stem Cells International / 2011 / Article / Fig 4

Research Article

An Inducible Expression System of the Calcium-Activated Potassium Channel 4 to Study the Differential Impact on Embryonic Stem Cells

Figure 4

Doxycycline treatment alters cell behavior. (a) Elongated dox treatment for 3 days alters the pluripotency and morphology of the ES cells. Oct3/4 proteins (red) are decreased, and green fluorescence is solely expressed by dox-treated cells. (b) In line, the ES cell marker SSEA1 is lost during dox treatment (red), and the actin cytoskeleton, visualized by the f-actin marker phalloidin, is altered upon dox treatment. (c) Proliferation of iSK4 cells is not changed by dox-treatment shown in a proliferation assay. ( đť‘› = 4 ) for all experiments. Nuclei are stained by DAPI (blue). Scale bars as indicated.

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