“Humanized” Stem Cell Culture Techniques: The Animal Serum Controversy

Table 3

Protein precursors abundant in UCBS and their possible functions in in vitro MSC-culture.

S. no.	Protein precursor	In Vitro effects on hMSCs
(1)	Alpha-2-macroglobulin precursor	(i) Natural protease inhibitor
		(ii) Role in cell regulation and differentiation
(2)	Apolipoprotein B-100 precursor	(i) Cholesterol transporter activity,
(3)	Complement C3 precursor	(i) Positive regulation of VEGF production
		(ii) G-protein coupled receptor protein signaling pathway
		(iii) Natural protease inhibitor
(4)	Complement C5 precursor	(i) Chemotaxis, positive regulation of VEGF production
		(ii) Natural protease inhibitor
		(iii) G-protein coupled receptor protein signaling pathway
		(iv) Positive regulation of chemokine production
(5)	Isoform 1 of complement factor H precursor	(i) Negative regulation of complement C3