Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood
Figure 2
Growth and erythroid maturation of MNC prospectively isolated on the basis of CD34/CD36 profiling under HEMA conditions. (a) Growth curve of cells prospectively isolated from AB MNC under HEMA conditions (the same color code as in Figure 1). MNC (grey dotted line) were cultured in parallel as control. The number of cells present in the cultures is expressed as fold increase (FI). Results from a representative experiment are shown. Similar results were observed in 2 additional experiments (see also Table 1). (b) Time course of the maturation of EBs in HEMA cultures seeded with either AB MNC or with populations A, B, C, or E, as indicated. EBs maturation was defined on the basis of CD36/CD235a profiling which divides EBs into three populations: (proerythroblasts, blue); (basophilic erythroblasts, purple), and (orthochromatic erythroblasts, red). Forward and side scatter analyzes identified a fourth population of small cells, probably represented by pyrenocytes (yellow). Cells which do not express EB markers are indicated in green. The numbers within each quadrant indicate the frequency of the different subpopulations. Results are representative of those obtained in three independent experiments. nd = not done, due to low cell numbers.
