Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NOD/SCID/IL2R mice
Figure 6
Significant level of extGLuc signal is detectable in splenectomized mice 24 hrs after transfusion. Bioluminescent imaging of nonsplenectomized (left panels) and splenectomized (right panels) mice 24 hrs after injection with extGLuc+ EBs, as indicated. Dorsal and ventral imaging is presented in the top and bottom panels, respectively. In intact mice, the bioluminescent signal was virtually undetectable other than at the site of injection (the tail). In the splenectomized mice, significant signal levels were observed in limbs (lower extremity), skull (indicating marrow localization), and abdomen (indicating liver localization because the spleen had been removed). Arrows indicate the positive signal. Data are representative of those observed with 4 mice per experimental point.
