Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NOD/SCID/IL2R mice
Figure 7
By flow cytometry of human CD36/CD235a expression, human cells were detectable in marrow and liver of splenectomized mice 4 days after transfusion. Flow cytometry analysis of bone marrow (left panels), spleen (middle panels) and liver (right panels) from splenectomized (top panels), non splenectomized (middle panels), and nontransfused controls (bottom panels) of representative mice are shown. The small numbers indicate the frequency of the cells detected within the respective quadrant. Values within circles correspond to the percentage of human cells calculated by subtracting the frequency detected in the negative controls in the corresponding bottom panels. The red circles indicate the frequency of human cells. Of note, the majority of the human CD235a positive cells were negative for CD36, an indication that the human EBs had matured in vivo. Similar results were obtained by analyzing the cells for eGFP expression. Data are representative of those obtained with 3 mice per experimental point.
