Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure

Figure 1

Morphology and growth characteristics of AF cells under different culture conditions. (a) AF cells cultivated in the presence of α-MEM supplemented with 10% FBS exhibit an elongated morphology. (b) In the presence of ADMEM with 5% FBS cells revealed a rather epithelioid shape. (c) Cells cultivated in α-MEM (10% FBS) showed a more homogenous generation doubling time (GDT) (d) compared with those cultivated in ADMEM (5% FBS) (𝑛=5). (e) The GDT of cells derived from amniocenteses is rather homogenous with an average survival time of 60–80 days. (f) Cells derived from amniodrainages reveal somewhat longer survival times than cells derived from amniocenteses and their growth dynamics are inhomogenous (𝑛=5). Scale bar in a and b = 25 μm.

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