Research Article

Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure

Figure 3

CFU assay for the demonstration and comparison of the self-renewing potential of CD117− and CD117+ AF-cells. Plating of very low cell numbers leads to the generation of single colonies (CFU) as demonstrated by the cresyl violet staining. (a) Colony formation after plating of 1000 CD117(−) cells per 100 mm culture dish. (b) Colony formation after plating of 1000 CD117(+) cells per dish. Scale bar = 1 cm.
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(a)
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(b)