Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure
Figure 7
Integration and differentiation of GFP labelled (green fluorescence) predifferentiated CD117− AF cells after grafting into the striatum of adult rats. (a) GFP labelled cells can be found around the injection site in the striatum. (b) one week after grafting GFP expressing cells are still arranged in clusters near the injection site, in a co staining with the astrocyte marker GFAP (Cy2 conjugated secondary antibody, red fluorescence) (c) and (d) typical neural cell morphologies of GFP expressing cells with elongated processes can be detected 2 weeks after grafting in a costaining with the early neuronal marker α-internexin (Cy2,red), (e) a dense network of GFP expressing processes are visible, (f) which show a co-staining with the astrocyte marker GFAP (Cy2, red). (g) Overlay of GFP (green) and GFAP (red) expressing cells. Scale bar in (a) = 20 μm, in (b) = 15 μm and in (c) and (d) = 10 μm.
