Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells

Table 1

qRTPCR analysis of differentiated rMAPC. Endothelium markers Flk1, Flt1, and Eg1 and liver markers HNF3b, AFP, and TTR were measured and Ct values normalized to GAPDH levels to determine ΔCt. Adult rat liver was used as the positive control and corresponding undifferentiated cells as negative control. Relative expression was carried out by determining ΔCt values and comparing expression levels to the positive control sample.

	FIk1			FIt1			Eg1
	ΔCt	SD	Rel Exp	ΔCt	SD	Rel Exp	ΔCt	SD	Rel Exp
MAPC	20.13	4.95	0.00006	15.23	11.81	0.00071	4.46	0.7	0.23982
Luc	8.34	0.35	0.22298	2.42	0.69	5.09824	1.79	0.69	1.52626
Int	10.13	0.15	0.0647	2.61	0.34	4.46915	2.28	0.33	1.08673
SB	8.84	0.63	0.15822	2.46	0.06	4.95883	1.8	0.13	1.51572
Adult rLiver	6.18	1.23	1	4.77	0.45	1	2.4	0.1	1
	HNF3b			AFP			TTR
	ΔCt	SD	Rel Exp	ΔCt	SD	Rel Exp	ΔCt	SD	Rel Exp
MAPC	3.27	0.21	1.38992	22.03	0.5	0	13.34	0.57	0
Luc	2.6	0.16	2.21914	16.42	0.32	0.0002	13.22	0.25	0
Int	2.51	0.14	2.35381	10.99	6.1	0.00867	3.95	1.96	0.0003
SB	1.78	0.35	3.90413	6.43	0.26	0.20448	1.95	0.2	0.0012
Adult rLiver	3.75	0.22	1	4.14	0.15	1	neg7.76	0.09	1

HNF3b: hepatocyte nuclear factor-3-beta; AFP: alpha-fetoprotein; TTR: transthyretin for liver differentiation, and Flk1: vascular endothelial growth factor receptor-2; Flt1: vascular endothelial growth factor receptor-1; Eg1: endothelial-derived gene-1 for differentiation into endothelium. Total RNA isolated from adult rat liver tissue (rLiver) was used as a positive control for liver and endothelial markers and undifferentiated MAPC (MAPC) used as the negative control.