Expression Patterns of Cancer-Testis Antigens in Human Embryonic Stem Cells and Their Cell Derivatives Indicate Lineage Tracks

Figure 2

CTA expression patterns in hES SC5, SC7, and SC3a cells and hEC PA-1 cells. (a) Activity of alkaline phosphatase and OCT4 in undifferentiated hES and hEC cells and in EBs formed by these cell lines. Scale bar = 100 μm. (b) Histological sections through teratomas and teratocarcinomas formed by hES and hEC cell lines. Cell derivatives of three germ layers were found in teratomas (TES) formed by hES cells and entirely cancer cells in teratocarcinomas (TEC). Scale bar = 100 μm. (c) Normal diploid karyotypes of hES SC5, SC7, and SC3a cells: SC5—46, XX; SC7—46, XY; SC3a—46, XX. (d) Expression of CTAs in hES SC5, SC7, and SC3a cells, hEC PA-1 cells and EBs formed by these cells.